新霉素ELISA快速檢測試劑盒(檢組織/乳制品，靈敏度0.5ppb，一步法)
新霉素ELISA快速檢測試劑盒
一、原理
本試劑盒采用間接競爭ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物新霉素將和微孔條上預(yù)包被的偶聯(lián)抗原競爭抗新霉素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物新霉素的含量成負(fù)相關(guān)，與標(biāo)準(zhǔn)曲線比較即可得出相應(yīng)殘留物新霉素的含量。
二、試劑盒特性
u 試劑盒靈敏度： 0.5ppb
u 孵育溫度： 25℃
u 孵育時間： 60min～15min
u 樣本檢測下限
組織（雞肉/肝、豬肉/肝、蝦、魚 ········· 10ppb
蜂 蜜 ········································· 10ppb
牛奶、奶粉······································· 10ppb
u 交叉反應(yīng)率
新霉素 ········································· 100%
鏈霉素 ········································· ﹤0.1%
慶大霉素 ······································ ﹤0.1%
雙氫鏈霉素 ··································· ﹤0.1%
卡那霉素 ······································ ﹤0.1%
妥布霉素 ······································ ﹤0.1%
紫蘇霉素 ······································ ﹤0.1%
u 樣本回收率
組織（雞/豬肉、蜂蜜、蝦、魚）·········· 85±19%
雞肝、豬肝 ··································· 70±17%
牛奶、奶粉 ··································· 85±16%
三、試劑盒組成
1 | 微量測試孔 | 每條8孔，一板12條 | |
2 | 標(biāo)準(zhǔn)液×6瓶 （1ml/瓶） | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | 酶標(biāo)記物 | 7ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
9 | 2X濃縮復(fù)溶液 | 50ml | 透明帽 |
四、樣品前處理及檢測步驟
詳見產(chǎn)品說明書。
五、儲藏條件和保質(zhì)期
1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。
2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見包裝盒。
